This study compared, for the first time, the postharvest conservative action of edible fungal chitosan coatings (gel, nanoparticles and gel-nanoparticle) on the physico-chemical, sensorial and microbiological characteristics of strawberries. The nanoparticles were prepared by an ionic gelation method and characterized by dynamic light scattering and scanning electron microscopy. The antioxidant (DPPH* and ABTS*) activity of the edible coatings and the antimicrobial (macrodilution method) action against phytopathogenic fungi were verified. The nanoparticles had a size of 331.1 nm and a zeta potential of+ 34 mV. The gel, nanoparticles and gel+nanoparticles exhibited minimum inhibitory concentration values ranging from 4 to 5, 1.5 to 2.5 and 1.0 + 0.5 to 2.0 + 1.5 g.L-1, respectively. All the edible coatings exhibited antifungal action. All the coatings had high scavenging activity, especially the gel edible coating. The coatings, especially the gel+nanoparticles, decreased the weight loss, microbiological growth, soluble solids, maturity index and moisture loss of the strawberry and preserved the pH values, anthocyanin content, titratable acidity and sensory characteristics. Therefore, the use of chitosan edible coating containing nanoparticles can be a promising strategy to improve the post-harvest quality of strawberries.
Ali A, Noh N, Mustafa M. Antimicrobial activity of chitosan enriched with lemongrass oil against anthracnose of bell pepper. Food Packaging And Shelf Life. 2015. p. 56–61.
2.
Allan C, Allan C, Hadwiger L. The fungicidal effect of chitosan on fungi of varying cell wall composition. 1979.
3.
Amarante C, Banks N, Ganesh S. Association of Official Analytical Chemists International. Official methods of analysis of AOAC international. Postharvest Biology and Technology. 2001. p. 291–301.
4.
Badawy M, Rabea E. A biopolymer chitosan and its derivatives as promising antimicrobial agents against plant pathogens and their applications in crop protection. International Journal of Carbohydrate Chemistry. 2011.
5.
Berger L, Stamford T, De Oliveira K, Pessoa A, De Lima M, Pintado M, et al.
6.
Souza E, Bugnicourt L, Alcouffe P, Ladavière C. Elaboration of chitosan nanoparticles: Favorable impact of a mild thermal treatment to obtain finely divided, spherical, and colloidally stable objects. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 2018. p. 476–86.
7.
Calvo P, Remunanlopez C, Vilajato J, Alonso M. Novel hydrophilic chitosan-polyethylene oxide nanoparticles as protein carriers. Journal of Applied Polymer Science. 1997. p. 125–32.
8.
Cao F, Guan C, Dai H, Li X, Zhang Z. Soluble solids content is positively correlated with phosphorus content in ripening strawberry fruits. Scientia Horticulturae. 2015. p. 183–7.
9.
Branco Melo C, De Mendoncasoares N, Diniz B, Leal K, Canto C, Flores D, et al. Effects of fungal chitosan nanoparticles as eco-friendly edible coatings on the quality of postharvest table grapes. Postharvest Biology And Technology. 2018. p. 56–66.
10.
Chen W, Li Y, Yang S, Yue L, Jiang Q, Xia W. Synthesis and antioxidant properties of chitosan and carboxymethyl chitosan-stabilized selenium nanoparticles. Carbohydrate Polymers. 2015. p. 574–81.
11.
Guerra D, Lima De Oliveira I, De Souza Pontes P, Suassuna Carneiro Lucio A, Tavares A, Barbosa-Filho J, et al. sential oils to prevent common postharvest mold infections and maintain the quality of cherry tomato fruit. International Journal Of Food Microbiology. 2015. p. 168–78.
12.
Dhital R, Joshi P, Becerra-Mora N, Umagiliyage A, Chai T, Kohli P, et al. Integrity of edible nanocoatings and its effects on quality of strawberries subjected to simulated in-transit vibrations. 2017. p. 257–64.
13.
Dos Santos N, Aguiar A, De Oliveira C, De Sales C, Silva S, Da Silva R, et al. Efficacy of the application of a coating composed of chitosan and Origanum vulgare l. essential oil to control Rhizopus stolonifer and Aspergillus niger in grapes. Vitis labrusca l.) Food Microbiology. 2012. p. 345–53.
14.
Eshghi S, Hashemi M, Mohammadi A, Badii F, Mohammadhoseini Z, Ahmadi K. Effect of nanochitosan-based coating with and without copper loaded on physicochemical and bioactive components of fresh strawberry fruit (Fragaria x ananassa duchesne) during storage. Food and Bioprocess Technology. 2014. p. 2397–409.
15.
Famiani F, Battistelli A, Moscatello S, Cruz-Castillo J, Walker R. The organic acids that are accumulated in the flesh of fruits: Occurrence, metabolism and factors affecting their contents-a review. Revista Chapingo Serie Horticultura. 2015. p. 97–128.
16.
Famiani F, Cultrera N, Battistelli A, Casulli V, Proietti P, Standardi A.
17.
Walker R. Phosphoenolpyruvate carboxykinase and its potential role in the catabolism of organic acids in the flesh of soft fruit during ripening. Journal of Experimental Botany. 2005. p. 2959–69.
18.
Fan W, Yan W, Xu Z, Ni H. Formation mechanism of monodisperse, low molecular weight chitosan nanoparticles by ionic gelation technique. Colloids and Surfaces B-biointerfaces. 2012. p. 21–7.
19.
Garcia-Rincon J, Vega-Perez J, Guerra-Sanchez M, Hernandez-Lauzardo A, Pena-Diaz A, Velazquez-Del, et al. Effect of chitosan on growth and plasma membrane properties of rhizopus stolonifer (ehrenb.:fr.) vuill. Pesticide Biochemistry and Physiology. 2010. p. 275–8.
20.
Garcia J, Medina R, Olias J. Quality of strawberries automatically packed in different plastic films. Journal of Food Science. 1998. p. 1037–41.
21.
Ghasemnezhad M, Nezhad M, Gerailoo S. Changes in postharvest quality of loquat (Eriobotrya japonica) fruits influenced by chitosan. Horticulture Environment and Biotechnology. 2011. p. 40–5.
22.
Gol N, Patel P, Rao T. Improvement of quality and shelflife of strawberries with edible coatings enriched with chitosan. Postharvest Biology And Technology. 2013. p. 185–95.
23.
Guerreiro A, Gago C, Faleiro M, Miguel M, Antunes M. The use of polysaccharide-based edible coatings enriched with essential oils to improve shelf-life of strawberries. Postharvest Biology and Technology. 2015. p. 51–60.
24.
Hajji S, Younes I, Affes S, Boufi S, Nasri M. Optimization of the formulation of chitosan edible coatings supplemented with carotenoproteins and their use for extending strawberries postharvest life. Food Hydrocolloids. 2018. p. 375–92.
25.
Kaewklin P, Siripatrawan U, Suwanagul A, Lee Y. Active packaging from chitosan-titanium dioxide nanocomposite film for prolonging storage life of tomato fruit. International Journal of Biological Macromolecules. 2018. p. 523–9.
26.
Khalifa I, Barakat H, El-Mansy H, Soliman S. Enhancing the keeping quality of fresh strawberry using chitosanincorporated olive processing wastes. Food Bioscience. 2016. p. 69–75.
27.
IJFS October. 2020. p. 373–93.
28.
Strawberries coated with fungal chitosan (gel, nanoparticles, gel + nanoparticles. p. 391.
29.
Kheiri A, Jorf S, Malihipour A, Saremi H, Nikkhah M. Application of chitosan and chitosan nanoparticles for the control of fusarium head blight of wheat (Fusarium graminearum) in vitro and greenhouse. International Journal of Biological Macromolecules. 2016. p. 1261–72.
30.
Kiilll C, Barud H, Santagneli S, Lima Ribeiro S, Silva A, Tercjak A. Synthesis and factorial design applied to a novel chitosan/sodium polyphosphate nanoparticles via ionotropic gelation as an RGD delivery system. Carbohydrate Polymers. 2017. p. 1695–702.
31.
Kong M, Chen X, Xing K, Park H. Antimicrobial properties chitosan and mode of action: A state of art review. International journal of food microbiology. 2010. p. 51–63.
32.
Larrauri J, Ruperez P, Sauracalixto F. Effect of drying temperature on the stability of polyphenols and antioxidant activity of red grape pomace peels. Journal of Agricultural and Food Chemistry. 1997. p. 1390–3.
33.
Lee S, Song K, Lee B. Antibacterial activity of silver nanoparticles prepared by a chemical reduction method. Korean Journal of Chemical Engineering. 2010. p. 688–92.
34.
Lees D, Francis F. Standardization of pigment analyses in cranberries. 1972.
35.
Ma Z, Garrido-Maestu A, Jeong K, Li B, Liu J, Tian S. Physiological responses and quality attributes of table grape fruit to chitosan preharvest spray and postharvest coating during storage. Carbohydrate polymers. 2017. p. 501–8.
36.
Mubarakali D, Lewisoscar F, Gopinath V, Alharbi N, Alharbi S, Thajuddin N. An inhibitory ac-tion of chitosan nanoparticles against pathogenic bacteria and fungi and their potential applications as biocompatible antioxidants. Microbial Pathogenesis. 2018. p. 323–7.
37.
Muzzarelli R, Boudrant J, Meyer D, Manno N, Demarchis M, Paoletti M. Current views on fungal chitin/chitosan, human chitinases, food preservation, glucans, pectins and inulin: A tribute to henri braconnot, precursor of the carbohydrate polymers science, on the chitin bicentennial. Carbohydrate Polymers. 2012. p. 995–1012.
38.
Oregel-Zamudio E, Angoa-Pérez M, Oyoque-Salcedo G, Aguilar-González C, Mena-Violante H. Effect of candelilla wax edible coatings combined with biocontrol bacteria on strawberry quality during the shelf-life. 2017.
39.
Scientia Horticulturae. p. 273–9.
40.
Paomephan P, Assavanig A, Chaturongakul S, Cady N, Bergkvist M, Niamsiri N. Insight into the antibacterial property of chitosan nanoparticles against Escherichia coli and Salmonella typhimurium and their application as vegetable wash disinfectant. Food Control. 2018. p. 294–301.
41.
Park Y, Kim MH, Park SC, Cheong H, Jang MK, Nah JW, et al. Investigation of the antifungal activity and mechanism of action of lmws-chitosan. Journal Microbiol Biotechnol. 2008. p. 1729–34.
42.
Perdones A, Sanchez-Gonzalez L, Chiralt A, Vargas M. Effect of chitosanlemon essential oil coatings on storagekeeping quality of strawberry. Postharvest Biology and Technology. 2012. p. 32–41.
43.
Pilon L, Spricigo P, Miranda M, De Moura M, Assis O, Mattoso L, et al. Chitosan nanoparticle coatings reduce microbial growth on fresh-cut apples while not affecting quality attributes. International Journal of Food Science & Technology. 2015. p. 440–8.
44.
IJFS October. 2020. p. 373–93.
45.
Saharan V, Sharma G, Yadav M, Choudhary M, Sharma S, Pal A, et al. Synthesis and in vitro antifungal efficacy of Cu-chitosan nanoparticles against pathogenic fungi of tomato. International Journal of Biological Macromolecules. 2015. p. 346–53.
46.
Severino P, Andreani T, Macedo A, Fangueiro J, Santana M, Silva A, et al. Current state-of-art and new trends on lipid nanoparticles (sln and nlc) for oral drug delivery. Journal of Drug Delivery. 2012.
47.
Shahbazi Y. Application of carboxymethyl cellulose and chitosan coatings containing Mentha spicata essential oil in fresh strawberries. International journal of biological macromolecules. 2018. p. 264–72.
48.
Sharma N, Tripathi A. Effects of Citrus sinensis (L.) Osbeck epicarp essential oil on growth and morphogenesis of Aspergillus niger. L.) Van Tieghem. Microbiological Research. 2008. p. 337–44.
49.
Siripatrawan U, Harte B. Physical properties and antioxidant activity of an active film from chitosan incorporated with green tea extract. Food Hydrocolloids. 2010. p. 770–5.
50.
Sullivan D, Cruz-Romero M, Collins T, Cummins E, Kerry J, Morris M. Synthesis of monodisperse chitosan nanoparticles. Food Hydrocolloids. 2018. p. 355–64.
51.
Tsai ML, Chen RH, Bai SW, Chen WY. 11th International Conference on Chitin and Chitosan (11th ICCC). Carbohydrate Polymers. 2011. p. 756–61.
52.
Valenzuela C, Tapia C, López L, Bunger A, Escalona V, Abugoch L. Effect of edible quinoa protein-chitosan based films on refrigerated strawberry (Fragaria x ananassa) quality. Electronic Journal of Biotechnology. 2015. p. 406–11.
53.
Vargas M, Albors A, Chiralt A, Gonzalez-Martinez C. Quality of cold-stored strawberries as affected by chitosan-oleic acid edible coatings. Postharvest Biology and Technology. 2006. p. 164–71.
54.
Vasconcelos De Oliveira C, Magnani M, De Sales C, De Souza Pontes A, Campos-Takaki G, Montenegro Stamford T, et al. Effects of chitosan from cunninghamella elegans on virulence of postharvest pathogenic fungi in table grapes. Vitis labrusca L.) International Journal of Food Microbiology. 2014. p. 54–61.
55.
Vasconcelos De Oliveira C, Magnani M, De Sales C, De Souza Pontes A, Campos-Takaki G, Montenegro Stamford T, et al. Bioactive compounds and antioxidant capacity of camarosa and selva strawberries. Food Microbiology. Fragaria x ananassa Duchesne). Foods; 2014. p. 120.
56.
Velickova E, Winkelhausen E, Kuzmanova S, Alves V, Moldao-Martins M. Impact of chitosan-beeswax edible coatings on the quality of fresh strawberries (Fragaria ananassa cv Camarosa) under commercial storage conditions. LWT -Food Science and Technology. 2013. p. 80–92.
57.
Ventura-Aguilar I, Bautista-Banos S, Flores-Garcia G, Zavaleta-Avejar L. Impact of chitosan based edible coatings functionalized with natural compounds on Colletotrichum fragariae development and the quality of strawberries. Food Chemistry. 2018. p. 142–9.
58.
Verlee A, Mincke S, Stevens C. Recent developments in antibacterial and antifungal chitosan and its deriva-IJFS October. 2017. p. 373–93.
59.
Strawberries coated with fungal chitosan (gel, nanoparticles, gel + nanoparticles) 393 tives. Carbohydrate Polymers. p. 268–83.
60.
Xing K, Shen X, Zhu X, Ju X, Miao X, Tian J, et al. Synthesis and in vitro antifungal efficacy of oleoyl-chitosan nanoparticles against plant pathogenic fungi. International Journal of Biological Macromolecules. 2016. p. 830–6.
61.
Yaman O, Bayoindirli L. Effects of an edible coating and cold storage on shelflife and quality of cherries. LWT -Food Science and Technology. 2002. p. 146–50.
62.
Yen MT, Yang JH, Mau JL. Antioxidant properties of chitosan from crab shells. Carbohydrate Polymers. 2008. p. 840–4.
63.
Yien L, Zin N, Sarwar A, Katas H. Antifungal activity of chitosan nanoparticles and correlation with their physical properties. International journal of Biomaterials. 2012.
64.
Yousuf B, Qadri O, Srivastava A. Recent developments in shelf-life extension of fresh-cut fruits and vegetables by application of different edible coatings: A review. 2018. p. 198–209.
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