Fermentation of tender coconut water by probiotic bacteria Bacillus coagulans

Aishwarya Singh Gangwar ,
Aishwarya Singh Gangwar

Department of Food Technology, Jamia Hamdard, New Delhi, India

Aastha Bhardwaj ,
Aastha Bhardwaj

Department of Food Technology, Jamia Hamdard, New Delhi, India

Vasudha Sharma
Vasudha Sharma
Contact Vasudha Sharma

Department of Food Technology, Jamia Hamdard, New Delhi, India

Published: 18.04.2018.

Volume 7, Issue 1 (2018)

pp. 100-110;

https://doi.org/10.7455/ijfs/7.1.2018.a9

Abstract

Coconut water is currently being considered as an elixir for patients suffering from diseases like dengue and malaria as well as chikungunia to provide hydration properties to the body. It has become a popular beverage for many people owing to its palatability and high mineral content. In this study, the growth, survival and fermentation performance of the probiotic bacterium Bacillus coagulans in coconut water was assessed in order to produce a novel non-dairy, probiotic beverage. The species was characterized on the basis of morphology, physiology and biochemical parameters and its probiotic attributes were assessed. Batch fermentations were carried out for 2 days at a constant 37°C, thereafter the samples were subjected to microbiological and chemical analysis. The results suggested that the specie produced lactic acid and was acid and bile tolerant. The pH and titratable acidity of probiotic fermented coconut water were found to be 4.4 and 0.53 % lactic acid, respectively. The viscosity of fermented coconut water increased significantly from an initial 5.13 mPa.s to 5.35 mPa.s because of the increase in soluble solids content due to exopolysaccharide production by B. coagulans during fermentation. Also, the overall acceptability score of probiotic coconut water was higher than tender coconut water, suggesting its feasibility for use as a probiotic beverage.

Keywords

References

1.
Acharya V, Gupta K, Golwala A, Store S, Sheth U. Comparative study of intravenous use of natural coconut water. synthetic coconut water and glucose saline in acute gastro-enteritis. Journal Of Medical Research. 1965. p. 1069–73.
2.
Aly S, Abd-El-Rahman A, John G, Mohamed M. Characterization of some bacteria isolated from oreochromis niloticus and their potential use as probiotics. Aquaculture. 2008. p. 1–6.
3.
Angelov A, Gotcheva V, Kuncheva R, Hristozova T. Development of a new oat-based probiotic drink. International Journal Of Food Microbiology. 2006. p. 75–80.
4.
Anurag P, Rajamohan T. Cardioprotective effect of tender coconut water in experimental myocardial infarction. Plant Foods for Human Nutrition. 2003. p. 1–12.
5.
Argyri A, Zoumpopoulou G, Karatzas KA, Tsakalidou E, Nychas GJ, Panagou E, et al. Selection of potential probiotic lactic acid bacteria from fermented olives by in vitro tests. Food Microbiology. 2013. p. 282–91.
6.
Campbell-Falck D, Thomas T, Falck T, Tutuo N, Clem K. The intravenous use of coconut water. American Journal Of Emergency Medicine. 2000. p. 90062–7.
7.
Chauhan O, Archana B, Singh A, Raju P, Bawa A. A refreshing beverage from mature coconut water blended with lemon juice. Journal Of Food Science And Technology-Mysore. 2014. p. 3355–61.
8.
Chung H, Kim Y, Chun S, Ji G. Screening and selection of acid and bile resistant bifidobacteria. International Journal Of Food Microbiology. 1999. p. 25–32.
9.
Cutting S. Bacillus probiotics. Food Microbiology. 2011. p. 214–20.
10.
Demirci A, Pometto A, Johnson K. Lactic acid production in a mixedculture biofilm reactor. Applied and Environmental Microbiology. 1993. p. 203–7.
11.
Dharmasena M. Assessment of viability of probiotic bacteria in non dairy food matrices under refrigeration storage (Doctoral dissertation. 2012.
12.
Duc L, Hong H, Barbosa T, Henriques A, Cutting S. Characterization of bacillus probiotics available for human use. Applied And Environmental Microbiology. 2004. p. 2161–71.
13.
Fao, Who. Health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria. FAO Food and Nutrition Paper; 2001.
14.
Gilliland S, Staley T, Bush L. Importance of bile tolerance of lactobacillus acidophilus used as a dietary adjunct1. Journal of Dairy Science. 1984. p. 81670–7.
15.
Hawrelak J. 266449396 Probiotics Choosing The Right One For Your Needs. Journal of the Australian Traditional -Medicine Society. 2003. p. 67–75.
16.
Hyronimus B, Le Marrec C, Sassi A, Deschamps A. Acid and bile tolerance of spore-forming lactic acid bacteria. International Journal Of Food Microbiology. 2000. p. 193–7.
17.
Jackson J, Gordon A, Wizzard G, Mc-Cook K, Rolle R. Changes in chemical composition of coconut (cocos nucifera) water during maturation of the fruit. Journal Of The Science Of Food And Agriculture. 2004. p. 1049–52.
18.
Jensen H, Grimmer S, Naterstad K, Axelsson L. In vitro testing of commercial and potential probiotic lactic acid bacteria. International Journal Of Food Microbiology. 2012. p. 216–22.
19.
Kalman D, Feldman S, Krieger D, Bloomer R. Comparison of coconut water and a carbohydrate-electrolyte sport drink on measures of hydration and physical performance in exercise-trained men. Journal Of The International Society Of Sports Nutrition. 2012. p. 9.
20.
Kandler O, Weiss N. Bergey’s manual of systematic bacteriology. Williams & Wilkin; 1986.
21.
Kodali V, Sen R. Antioxidant and free radical scavenging activities of an exopolysaccharide from a probiotic bacterium. Biotechnology Journal. 2008. p. 245–51.
22.
Kuswardani I, Kusumawati N, Srianta I, Sabrina M. The 3rd International Conference of Indonesian Society for Lactic Acid Bacteria (3rd IC-ISLAB): Better Life with Lactic Acid Bacteria: Exploring Novel Functions of Lactic Acid Bacteria, held at Yogyakarta. ISLAB; 2011. p. 2–10.
23.
Lee PR, Boo C, Liu SQ. Fermentation of coconut water by probiotic strains lactobacillus acidophilus l10 and lactobacillus casei l26. Annals Of Microbiology. 2013. p. 1441–50.
24.
Lorca G, Valdez G. Acid tolerance mediated by membrane atpases in lactobacillus acidophilus. Biotechnology Letters. 2001. p. 777–80.
25.
Miller G. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry. 1959. p. 426–8.
26.
Payot T, Chemaly Z, Fick M. Lactic acid production by bacillus coagulanskinetic studies and optimization of culture medium for batch and continuous fermentations. Enzyme And Microbial Technol-ogy. 1999. p. 191–9.
27.
Penna A, Rao-Gurram S, Barbosa-Canovas G. Effect of milk treatment on acidification, physicochemical characteristics, and probiotic cell counts in low fat yogurt. Milchwissenschaft-Milk Science International. 2007. p. 48–52.
28.
Pennacchia C, Ercolini D, Blaiotta G, Pepe O, Mauriello G, Villani F. Selection of lactobacillus strains from fermented sausages for their potential use as probiotics. Meat Science. 2004. p. 309–17.
29.
Prado F, Lindner J, Inaba J, Thomaz-Soccol V, Bray S, Soccol C. Development and evaluation of a fermented coconut water beverage with potential health benefits. Journal Of Functional Foods. 2015. p. 489–97.
30.
Prasad J, Gill H, Smart J, Gopal P. Selection and characterisation of lactobacillus and bifidobacterium strains for use as probiotics. International Dairy Journal. 1998. p. 993–1002.
31.
Pummer S, Heil P, Maleck W, Petroianu G. Influence of coconut water on hemostasis. American Journal Of Emergency Medicine. 2001. p. 287–9.
32.
Ranadheera R, Baines S, Adams M. Importance of food in probiotic efficacy. Food Research International. 2010. p. 1–7.
33.
Ranganna S. Handbook of analysis and quality control for fruit and vegetable products. Tata McGraw Hill Publishing Co. Ltd; 1986. p. 0.
34.
Saat M, Singh R, Sirisinghe R, Nawawi M. Rehydration after exercise with fresh young coconut water, carbohydrateelectrolyte beverage and plain water. Journal Of Physiological Anthropology And Applied Human Science. 2002. p. 93–104.
35.
Sanders M. Probiotics. Food Technology. 1999. p. 67–77.
36.
Yong J, Ge L, Ng Y, Tan S. The chemical composition and biological properties of coconut (cocos nucifera l.) water. Molecules. 2009. p. 100–10.

Citation

Copyright

Article metrics

Google scholar: See link

The statements, opinions and data contained in the journal are solely those of the individual authors and contributors and not of the publisher and the editor(s). We stay neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Most read articles

Indexed by