Evaluation of test-kits for the detection of Escherichia coli O157 in raw meats and cattle faeces.

Hilda Nyati ,
Hilda Nyati
Contact Hilda Nyati

Food Safety Authority (VWA) , Zutphen , Netherlands

National University of Science and Technology , Bulawayo , Zimbabwe

Annet Heuvelink ,
Annet Heuvelink

Food Safety Authority (VWA) , Zutphen , Netherlands

Caroliene Van Heerwaarden ,
Caroliene Van Heerwaarden

Food Safety Authority (VWA) , Zutphen , Netherlands

Ans Zwartkruis
Ans Zwartkruis

Food Safety Authority (VWA) , Zuthpen , Netherlands

Published: 18.10.2012.

Volume 1, Issue 2 (2012)

pp. 126-134;

https://doi.org/10.7455/ijfs.v1i2.49

Abstract

Escherichia coli O157 detection limits in artificially contaminated beef and cattle faeces samples were determined using Dynabeads anti E. coli O157 immunomagnetic beads, VIDAS-UP, VIDAS-ICE, and real-time PCR (GeneDisc and LightCycler) systems. Dynabeads anti-E. coli O157 immunomagnetic separation (IMS) and the GeneDisc cycler were the most sensitive methods, and could detect an initial 1 CFU in 25g beef samples after 6h of incubation in modified tryptone soya broth with novobiocin (mTSB+n) or buffered peptone water (BPW). The VIDAS-UP method could detect an initial 10 CFU, while VIDAS-ICE and the LightCycler methods could only detect an initial 100 CFU. Higher detection rates were achieved with 18 hour incubations, where an initial 1 CFU in a 25g sample could be detected with all five methods. For cattle faeces enrichments, Dynabeads anti-E. coli O157 IMS could detect an initial 1 CFU after a 6 h incubation in mTSB+n, while the VIDAS-UP and VIDAS-ICE methods could detect an initial 10 CFU and both PCR methods could only detect an initial 100 CFU. Detection rates were lower in BPW, compared to mTSB+n, with thresholds of 100 CFU for VIDAS-ICE, VIDAS-UP and GeneDisc methods, and >100 CFU for the LightCycler method.

Keywords

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