This study investigated the ability of Lactobacillus plantarum strains (NGL5 and NGL7) and Candida tropicalis (NGY1) previously identified from akamu-a Nigerian fermented maize food with probiotic L. plantarum LpTx and Saccharomyces boulardii SB20 to ferment ground maize slurries based on pH, acidity, microbial biomass, levels of sugars and organic acids, and their antimicrobial activity against Salmonella enterica serovar Enteritidis NCTC 5188, Escherichia coli NCTC 11560, Bacillus cereus NCIMB 11925, Staphylococcus aureus NCTC 3750 and Listeria monocytogenes NCTC 7973 using an agar spot assay. L. plantarum strains either as single or mixed starter cultures with the yeasts had growth rates ≥0.15 h−1 , with pH significantly (p≤0.05) decreased to ≤3.93 after 12 h and then to ≤3.52 after 72 h and lactic acid >84 mmol L−1 . The yeasts had growth rates ≥0.18 h−1 but pH was ≥4.57 with lactic acid levels ≤20.23 mmol L−1 after 72 h in the single culture fermentation. There was no inhibition in modified MRS agar: 0.2% glucose and 0.2% glucose without Tween 80. Inhibition halos in MRS agar varied from 10.6 to 23.9 mm. S. bourladii was more inhibitory towards L. monocytogenes (8.6 mm) and B. cereus (5.4 mm ) than was C. tropicalis (1.1 and 3.3 mm for L. monocytogenes NCTC 7973 and B. cereus NCIMB 11925 respectively) (0.9 mm) in malt extract agar. This study showed that C. tropicalis was less inhibitory to the pathogens while antimicrobial activities of the L. plantarum strains were mainly due to acidity and the L. plantarum strains either as single or mixed cultures with the yeasts demonstrated strong fermentation ability, with significant decrease in pH which is vital in the choice of starter for product safety
Aidoo K, Nout M, Sarkar P. Occurrence and function of yeasts in asian indigenous fermented foods. Fems Yeast Research. 2006. p. 30–9.
2.
Akinrele I. Fermentation studies on maize during the preparation of a traditional african starch-cake food. Journal of the Science of Food and Agriculture. 1970. p. 619–25.
3.
Annan N, Poll L, Sefa-Dedeh S, Plahar W, Jakobsen M. Volatile compounds produced by Lactobacillus fermentum, Saccharomyces cerevisiae and Candida krusei in single starter culture fermentations of ghanaian maize dough. Journal of Applied Microbiology. 2003. p. 462–74.
4.
Banigo E, Muller H. Manufacture of ogi (a nigerian fermented cereal porridge) -comparative evaluation of corn, sorghum and millet. Canadian Institute of Food Science and. Technology Journal-Journal de L’Institut Canadien de Science et Technologie Alimentaires. 1972. p. 217–21.
5.
Baranyi J, Roberts T. A dynamic approach to predicting bacterial-growth in food. International Journal of Dairy Technology. 1994. p. 277–94.
6.
Edema M, Sanni A. Functional properties of selected starter cultures for sour maize bread. Food Microbiology. 2008. p. 616–25.
7.
Farthing M, Salam M, Lindberg G, Dite P, Khalif I, Salazar-Lindo E, et al. Acute diarrhea in adults and children: a global perspective. World Gastroenterology Organisation Global Guidelines. 2013.
8.
Ginovart M, Prats C, Portell X, Silbert M. International Conference of Predictive Modeling in Foods (6ICPMF). Food Microbiology. 2011. p. 810–7.
9.
Gobbetti M, Corsetti A. Lactobacillus sanfrancisco a key sourdough lactic acid bacterium: a review. Food Microbiology. 1997. p. 175–87.
10.
Halm M, Hornbaek T, Arneborg N, Sefa-Dedeh S, Jespersen L. Lactic acid tolerance determined by measurement of intracellular ph of single cells of candida krusei and saccharomyces cerevisiae isolated from fermented maize dough. International Journal of Dairy Technology. 2004. p. 97–103.
11.
Holzapfel W. Annual Meeting of the Institute-of-Food-Technologists. International Journal of Dairy Technology. Kempthall, Switzerland; 2002. p. 197–212.
12.
Kalui C, Mathara J, Kutima P, Kiiyukia C, Wongo L. Functional characteristics of Lactobacillus plantarum and Lactobacillus rhamnosus from ikii, a kenyan traditional fermented maize porridge. African Journal of Biotechnology. 2009. p. 4363–73.
13.
Kunene N, Hastings J, Holy A. Bacterial populations associated with a sorghum-based fermented weaning cereal. International Journal of Dairy Technology. 1999. p. 75–83.
14.
Lee CH. Food Science and Technology. Blackwell Publishing Ltd; 2009.
15.
Leroi F, Pidoux M. Characterization of interactions between lactobacillushilgardii and saccharomyces-florentinus isolated from sugary kefir grains. Journal of Applied Bacteriology. 1993. p. 54–60.
16.
Majhenic A, Lorberg P, Rogelj I. Characterisation of the Lactobacillus community in traditional karst ewe’s cheese. International Journal of Dairy Technology. 2007. p. 182–90.
17.
Miles A, Misra S, Irwin J. The estimation of the bactericidal power of the blood. Journal of Hygiene. 1938. p. 732–49.
18.
Motarjemi Y. Annual Meeting of the Institute-of-Food-Technologists. International Journal of Dairy Technology. KEMPTHALL, SWITZER-LAND; 2002. p. 213–29.
19.
Mugula J, Narvhus J, Sorhaug T. Use of starter cultures of lactic acid bacteria and yeasts in the preparation of togwa, a tanzanian fermented food. International Journal of Dairy Technology. 2003. p. 307–18.
20.
Murphy A, Kavanagh K. Emergence of saccharomyces cerevisiae as a human pathogen implications for biotechnology. Enzyme and Microbial Technology. 1999. p. 551–7.
21.
Muyanja C, Narvhus J, Treimo J, Langsrud T. Isolation, characterisation and identification of lactic acid bacteria from bushera: a ugandan traditional fermented beverage. International Journal of Dairy Technology. 2003. p. 201–10.
22.
Nguyen T, Loiseau G, Icard-Verniere C, Rochette I, Treche S, Guyot JP. Effect of fermentation by amylolytic lactic acid bacteria, in process combinations, on characteristics of rice/soybean slurries: a new method for preparing high energy density complementary foods for young children. Food Chem-istry. 2007. p. 623–31.
23.
Niven S, Beal J, Brooks P. The simultaneous determination of short chain fatty acid, monosaccharides and ethanol in fermented liquid pig diets. Animal Feed Science and Technology. 2004. p. 339–45.
24.
Nyatoti V, Mtero S, Rukure G. Pathogenic escherichia coli in traditional african weaning foods. Food Control. 1997. p. 51–4.
25.
Obinna-Echem P, Kuri V, Beal J. Journal of The Science of Food and Agriculture. 2014. p. 331–40.
26.
Omemu A, Oyewole O, Bankole M. Significance of yeasts in the fermentation of maize for ogi production. Food Microbiology. 2007. p. 571–6.
27.
Ouoba L, Diawara B, Jespersen L, Jakobsen M. Antimicrobial activity of Bacillus subtilis and Bacillus pumilus during the fermentation of african locust bean (parkia biglobosa) for Soumbala production. Journal of Applied Microbiology. 2007. p. 963–70.
28.
Reddy G, Altaf M, Naveena B, Venkateshwar M, Kumar E. Amylolytic bacterial lactic acid fermentation -a review. Biotechnology Advances. 2008. p. 22–34.
29.
Sezonov G, Joseleau-Petit D, Ari R. Escherichia coli physiology in luriabertani broth. Journal of Bacteriology. 2007. p. 8746–9.
30.
Shetty P, Jespersen L. Saccharomyces cerevisiae and lactic acid bacteria as potential mycotoxin decontaminating agents. Trends in Food Science & Technology. 2006. p. 48–55.
31.
Swinnen I, Bernaerts K, Dens E, Geeraerd A, Van Impe J. Predictive modelling of the microbial lag phase: a review. International Journal of Dairy Technology. 2004. p. 137–59.
32.
Tasteyre A, Barc M, Karjalainen T, Bourlioux P, Collignon A. Inhibition of in vitro cell adherence of clostridium difficile by saccharomyces boulardii. Microbial Pathogenesis. 2002. p. 219–25.
33.
Teniola O, Odunfa S. The effects of processing methods on the levels of lysine, methionine and the general acceptability of ogi processed using starter cultures. International Journal of Dairy Technology. 2001. p. 1–9.
34.
Teniola O, Odunfa S. Microbial assessment and quality evaluation of ogi during spoilage. World Journal of Microbiology & Biotechnology. 2002. p. 731–7.
35.
Towo E, Matuschek E, Svanberg U. Fermentation and enzyme treatment of tannin sorghum gruels: effects on phenolic compounds, phytate and in vitro accessible iron. Food Chemistry. 2006. p. 369–76.
36.
Trias R, Baneras L, Badosa E, Montesinos E. Bioprotection of golden delicious apples and iceberg lettuce against foodborne bacterial pathogens by lactic acid bacteria. International Journal of Dairy Technology. 2008. p. 50–60.
37.
Wakil S, Onilude A, Adetutu E, Ball A. PCR-DGGE fingerprints of microbial successional changes during fermentation of cereal-legume weaning foods. African Journal of Biotechnology. 2008. p. 4643–52.
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