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Cytotoxic, antioxidant and antimicrobial properties of red sweet pepper (Capsicum annuum L. var. Llanerón) extracts: In vitro study

Rosa Raybaudi-Massilia ,
Rosa Raybaudi-Massilia
Contact Rosa Raybaudi-Massilia

Institute of Food Science and Technology, Faculty of Science, Central University of Venezuela , Caracas , Venezuela

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Alırica I. Suarez ,
Alırica I. Suarez

Laboratory of Natural Products, Faculty of Pharmacy, University Central of Venezuela Venezuela

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Francisco Arvelo ,
Francisco Arvelo

Foundation Institute for Advanced Studies-IDEA , Caracas , Venezuela

Institute of Experimental Biology, Faculty of Science, Central University of Venezuela , Caracas , Venezuela

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Alexandra Zambrano ,
Alexandra Zambrano

Institute of Food Science and Technology, Faculty of Science, Central University of Venezuela , Caracas , Venezuela

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Felipe Sojo ,
Felipe Sojo

Foundation Institute for Advanced Studies-IDEA , Caracas , Venezuela

Institute of Experimental Biology, Faculty of Science, Central University of Venezuela , Caracas , Venezuela

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Marıa I. Calderon-Gabaldon ,
Marıa I. Calderon-Gabaldon

Institute of Food Science and Technology, Faculty of Science, Central University of Venezuela , Caracas , Venezuela

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Jonathan Mosqueda-Melgar
Jonathan Mosqueda-Melgar

Institute of Food Science and Technology, Faculty of Science, Central University of Venezuela , Caracas , Venezuela

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Published: 18.10.2017.

Volume 6, Issue 2 (2017)

pp. 222-231;

https://doi.org/10.7455/ijfs/6.2.2017.a8

Abstract

Alcoholic and aqueous extracts were obtained from red sweet pepper (Capsicum annuum L.) by different methodologies to evaluate their cytotoxic, antioxidant and antimicrobial properties. Alcoholic extracts (MFP, MSd, SFP, SDP, SSd) from fresh red sweet pepper (FP) and dry pulp (DP) and seed (Sd) were obtained by maceration (M) and Soxhlet (S) equipment using methanol as extraction solvent; whereas aqueous extracts (LFP, LSd) were obtained by decoction followed by lyophilization (L). Human tumoral cell lines from breast (MCF-7 and SKBr3), prostate (PC3) and cervix (HeLa), and fibroblasts (as control) were used to determine the cytotoxic properties by the MTT assay. Antioxidant and antimicrobial properties were determined by DPPH and disc diffusion method, respectively. The extracts SDP and SFP showed the higher cytotoxic activity. The SDP extract had a significant (P < 0.05) in-vitro effect on HeLa (1.9 ± 1.4 µg/mL) and PC3 (< 1 µg/mL) cells with a moderated impact on fibroblasts (26.1 ± 1.2 µg/mL); whereas, SFP had a significant (p < 0.05) effect on MCF-7 cell line (2.1 ± 1.2 µg/mL) with a moderated impact on fibroblasts (25.9 ± 1.0 µg/mL). The higher antioxidant activity was found for MFP (80.3 ± 0.2%) and SFP extracts (75.5 ± 0.5%). Mild antimicrobial activity was only observed for alcoholic extracts. The results showed the potential of red sweet pepper (C. annuum L.) as a source of antioxidant and cytotoxic compounds, and suggest the need of further studies to isolate and characterize the bioactive compounds that impart those properties. 

Keywords

Cytotoxic,

Antioxidant,

Antimicrobial,

Capsicum annuum L.,

Red sweet pepper

References

1.
Kobata K, Sutoh K, Todo T, Yazawa S, Iwai K, Watanabe T. Nordihydrocapsiate, a new capsinoid from the fruits of a nonpungent pepper, Capsicum annuum. Journal of Natural Products. 1999;(2):335–6.
2.
Yazdizadeh Shotorban N, Jamei R, Heidari R. Antioxidant activities of two sweet pepper Capsicum annuum l. varieties phenolic extracts and the effects of thermal treatment. Avicenna Journal of Phytomedicine. 2012;(1):25–34.
3.
Watjen W, Chovolou Y, Kampkötter A, Kahl R. Anti and Prooxidative effects of flavonoids. 2007;1–16.
4.
Suarez A, Blanco Z, Compagnone R, Salazar-Bookaman M, Zapata V, Alvarado C. Anti-inflammatory activity of Croton cuneatus aqueous extract. Journal of Ethnopharmacology. 2006;(1–2):99–101.
5.
Shaha R, Rahman S, Asrul A. Bioactive compounds in chilli peppers (Capsicum annuum l.) at various ripening (green, yellow and red) stages. Annals of Biological Research. 2013;(8):27–34.
6.
Padilha H, Pereira E, Munhoz P, Vizzotto M, Valgas R, Barbieri R. Genetic variability for synthesis of bioactive compounds in peppers (Capsicum annuum) from brazil. Food Science and Technology. 2015;(3):516–23.
7.
Omolo M, Wong ZZ, Mergen K, Hastings J, Le N, Reil H, et al. Antimicrobial properties of chili peppers. Journal of Infectious Diseases and Therapy. 2014;(4):145.
8.
Oboh G, Rocha J. Hot pepper (Capsicum spp.) protects brain from sodium nitroprusside-and quinolinic acidinduced oxidative stress in vitro. Journal of Medicinal Food. 2008;(2):349–55.
9.
Oboh G, Rocha J. Antioxidants in food: A new challenge for food processors. 2007;35–64.
10.
Nazzaro F, Caliendo G, Arnesi G, Veronesi A, Sarzi P, Fratianni F. Comparative content of some bioactive compounds in two varieties of Capsicum annuum l. sweet pepper and evaluation of their antimicrobial and mutagenic activities. Journal of Food Biochemistry. 2009;(6):852–68.
11.
Motohashi N, Wakabayashi H, Kurihara T, Takada Y, Maruyama S, Sakagami H, et al. Cytotoxic and multidrug resistance reversal activity of a vegetable. Phytotherapy Research. 2003;(4):348–52.
12.
Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods. 1983;(1):55–63.
13.
Materska M, Perucka I. Antioxidant activity of the main phenolic compounds isolated from hot pepper fruit (Capsicum annuuml. Journal of Agricultural and Food Chemistry. 2005;(5):1750–6.
14.
Leong K, Alviarez P, Compagnone R, Suarez A. Isolation and structural elucidation of chemical constituents of Amanoa almerindae. Pharmaceutical Biology. 2009;(6):496–9.
15.
Akhtar F, Sharif H, Mallick M, Zahoor F, Abdulmalik A, Baig W, et al. Capsaicin: its biological activities and in silico target fishing. Acta Poloniae Pharmaceutica. Drug Research. 2017;(2):321–9.
16.
Hernandez-Ortega M, Ortiz-Moreno A, Hernandez-Navarro M, Chamorro-Cevallos G, Dorantes-Alvarez L, Necoechea-Mondragon H. Antioxidant, antinociceptive, and anti-inflammatory effects of carotenoids extracted from dried pepper. Capsicum annuum l) Journal of Biomedicine and Biotechnology. 2012;1–10.
17.
Gonzalez-Aguilar G, Robles-Sanchez R, Martinez-Tellez M, Olivas G, Alvarez-Parrilla E, De La, et al. Bioactive compounds in fruits: health benefits and effect of storage conditions. Stewart Postharvest Review. 2008;(3):1–10.
18.
Diz M, Carvalho A, Rodrigues R, Neves-Ferreira A, Da Cunha M, Alves E, et al. Antimicrobial peptides from chilli pepper seeds causes yeast plasma membrane permeabilization and inhibits the acidification of the medium by yeast cells. Biochimica Et Biophysica Acta-general Subjects. 2006;(9):1323–32.
19.
Davidson P, Taylor T. Food microbiology: fundamentals and frontiers. 2007;713–45.
20.
Davidson P, Parish M. Methods for testing the efficacy of food antimicrobials. Food Technology. 1989;(1):148–55.
21.
Ciulu-Costinescu F, Chifiriuc M, Popa M, Bleotu C, Neamtu J, Averis L, et al. Screening of polyphenol content and in vitro studies of antioxidant, antibacterial and cytotoxic activities of Capsicum Annuum extracts. Revista De Chimie. 2015;(9):1261–6.
22.
Suarez A. Natural product communications. (Chap. Crotofolane Diterpenoids from Croton caracasanus. 2013;1679–82.
23.
Chavez K, Compagnone R, Riina R, Briceno A, Gonzalez T, Squitieri E.
24.
Celep E, Aydin A, Yesilada E. A comparative study on the in vitro antioxidant potentials of three edible fruits: cornelian cherry, japanese persimmon and cherry laurel. Food and Chemical Toxicol-ogy. 2012;(9):3329–35.
25.
Callacondo-Riva D, Quispe-Mauricio A, Lindo-Gamarra S, Vaisberg A. Cytotoxic activity of ethanolic extract of Gnaphalium spicatum keto keto on human tumor cell lines. Revista Peruana de Medicina Experimental y Salud Publica. 2008;(4):380–5.
26.
Ayala-Zavala J, Vega-Vega V, Rosas-Dominguez C, Palafox-Carlos H, Villa-Rodriguez J, Siddiqui M, et al. Agroindustrial potential of exotic fruit byproducts as a source of food additives. Food Research International. 2011;(7):1866–74.
27.
Alberto M, Canavosio M, De Nadra M. Antimicrobial effect of polyphenols from apple skins on human bacterial pathogens. Electronic Journal of Biotechnology. 2006;(3):3–4.

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