The aim of this study was to evaluate the survival of Lactobacillus acidophilus that had been simple or double spray dried using chitosan to cause microencapsulation and which had been exposed to model gastrointestinal conditions. In addition, the study also determined the physicochemical properties of the powder containing the microencapsulated probiotic. Chitosan-inulin or chitosan-maltodextrin (1:15 or 1:25) solutions were inoculated with 1012 CFU mL−1 of L. acidophilus, for simple microencapsulation. The different solutions were dried using a spray dryer with an inlet air temperature of 130 ◦C and a solution flux of 4.8 g min−1 . A two-step process was used for the double microencapsulation. In the first step, the probiotic was added to a gelatin-maltodextrin (1:25) solution and then spray dried; for the second step, the microencapsulated probiotic was added to a chitosan-inulin or chitosan-maltodextrin (1:25) solution and then it was spray dried again. With the simple microencapsulated probiotic, a microbial reduction of 7 log cycles was obtained. With the double microencapsulated probiotic only 3 log reductions were achieved. The double microencapsulated probiotic thus demonstrated greater resistance to simulated gastrointestinal conditions. The powders produced were shown to have water activity values of 0.176 - 0.261 at 25 ◦C and moisture content of 0.8 – 1.0%, which are characteristic of spray dried products. The bulk density was significantly (p < 0.05) lower (300 kg m−3 ) for simple than for double (400 kg m−3 ) microencapsulated probiotic powders. Solubility and dispersibility of the powder microcapsules were better at lower pH values. Double microencapsulation using a process of spray drying is therefore recommended for probiotics, thus exploiting chitosan’s insolubility in water, which can be applied for the of development food products.
Amin T, Thakur M, Jain S. Microencapsulation-the future of probiotic cultures. Journal of Microbiology, Biotechnology and Food Sciences. 2013;(1):35–43.
3.
Anal A, Singh H. Recent advances in microencapsulation of probiotics for industrial applications and targeted delivery. Trends in Food Science & Technology. 2007;(5):240–51.
4.
Ananta E, Volkert M, Knorr D. Cellular injuries and storage stability of spray-dried lactobacillus rhamnosus gg. International Dairy Journal. 2005;(4):399–409.
5.
Aoac. Official methods of analysis. 2000;(17).
6.
Avila-Reyes S, Garcia-Suarez F, Jimenez T, San Martin-Gonzalez M, Bello-Perez M, L. Protection of l. rhamnosus by spray-drying using two prebiotics colloids to enhance the viability. Carbohydrate Polymers. 2014;423–30.
7.
Barboza Y, Marquez E, Parra K, Patricia Pinero M, Medina L. Development of a potential functional food prepared with pigeon pea (cajanus cajan), oats and lactobacillus reuteri atcc 55730. International Journal of Food Sciences and IJFS October. 2012;188–200.
8.
Nutrition. (7):813–20.
9.
Chávez B, Ledeboer A. Drying of probiotics: optimization of formulation and process to enhance storage survival. Drying Technology. 2007;(7–8):1193–201.
10.
Chen K, Chen M, Liu J, Lin C, Chiu H. Optimization of incorporated prebiotics as coating materials for probiotic microencapsulation. Journal of Food Science. 2005;(5):260-M266.
11.
Corcoran B, Ross R, Fitzgerald G, Stanton C. Comparative survival of probiotic lactobacilli spray-dried in the presence of prebiotic substances. Journal of Applied Microbiology. 2004;(5):1024–39.
12.
Corona-Hernandez R, Alvarez-Parrilla E, Lizardi-Mendoza J, Islas-Rubio A, De La Rosa L, Wall-Medrano A. Structural stability and viability of microencapsulated probiotic bacteria: a review. Comprehensive Reviews in Food Science and Food Safety. 2013;(6):614–28.
13.
Desai K, Park H. Recent developments in microencapsulation of food ingredients. Drying Technology. 2005;(7):1361–94.
14.
Desmond C, Ross R, O’callaghan E, Fitzgerald G, Stanton C. Improved survival of lactobacillus paracasei nfbc 338 in spray-dried powders containing gum acacia. Journal of Applied Microbiology. 2002;(6):1003–11.
15.
Di Criscio T, Fratianni A, Mignogna R, Cinquanta L, Coppola R, Sorrentino E, et al. Production of functional probiotic, prebiotic, and synbiotic ice creams. Journal of Dairy Science. 2010;(10):4555–64.
16.
Ding W, Shah N. Acid, bile, and heat tolerance of free and microencapsulated probiotic bacteria. Journal of Food Science. 2007;(9):446-M450.
17.
Estevinho B, Rocha F, Santos L, Alves A. Microencapsulation with chitosan by spray drying for industr applications a review. Trends in Food Science & Technology. 2013;(2):138–55.
18.
Fritzen-Freire C, Prudencio E, Amboni R, Pinto S, Negrao-Murakami A, Murakami F. Microencapsulation of bifidobacteria by spray drying in the presence of prebiotics. Food Research International. 2012;(1):306–12.
19.
Fuchs M, Turchiuli C, Bohin M, Cuvelier M, Ordonnaud C, Peyrat-Maillard M, et al. Encapsulation of oil in powder using spray drying and fluidised bed agglomeration. Journal of Food Engineering. 2006;(1):27–35.
20.
Gawkowski D, Chikindas M. Nondairy probiotic beverages: the next step into human health. Beneficial Microbes. 2013;(2):127–42.
21.
Gbassi G, Vandamme T. Probiotic encapsulation technology: from microencapsulation to release into the gut. Pharmaceutics. 2012;(1):149–63.
22.
Gharsallaoui A, Roudaut G, Chambin O, Voilley A, Saurel R. Applications of spray-drying in microencapsulation of food ingredients: an overview. Food Research International. 2007;(9):1107–21.
23.
Granato D, Branco G, Nazzaro F, Cruz A, Faria J. Functional foods and nondairy probiotic food development: trends, concepts, and products. Comprehensive Reviews in Food Science and Food Safety. 2010;(3):292–302.
24.
Hernández-Carranza P, López-Malo A, Jiménez-Munguía MT. Microencapsulation quality and efficiency of lactobacillus casei by spray drying using maltodextrin and vegetable extracts. Journal of Food Research. 2013;(1):61–9.
25.
Ivanovska T, Petrusevska-Tozi L, Kostoska M, Geskovski N, Grozdanov A, Stain C, et al. Microencapsulation of lactobacillus casei IJFS October. 2012;200.
26.
Flores-Belmont. in chitosan-ca-alginate microparticles using spray-drying method. Macedonian Journal Of Chemistry And Chemical Engineering. (1):115–23.
27.
Kailasapathy K. Survival of free and encapsulated probiotic bacteria and their effect on the sensory properties of yoghurt. 2006;(10):1221–7.
28.
Karimi R, Mortazavian A, Da Cruz A. Viability of probiotic microorganisms in cheese during production and storage: a review. Dairy Science & Technology. 2011;(3):283–308.
29.
Lee J, Cha D, Park H. Survival of freeze-dried lactobacillus bulgaricus kfri 673 in chitosan-coated calcium alginate microparticles. Journal of Agricultural and Food Chemistry. 2004;(24):7300–5.
30.
Rokka S, Rantamaki P. Protecting probiotic bacteria by microencapsulation: challenges for industrial applications. European Food Research and Technology. 2010;(1):1–12.
31.
Schubert H. Food particle technology. part i: properties of particles and particulate food systems. Journal of Food Engineering. 1987;(1):90019–26.
32.
Telang A, Thorat B. Optimization of process parameters for spray drying of fermented soy milk. Drying Technology. 2010;(12):1445–56.
33.
Teoh P, Mirhosseini H, Mustafa S, Hussin A, Manap M. Recent approaches in the development of encapsulated delivery systems for probiotics. Food Biotechnology. 2011;(1):77–101.
34.
Urbanska A, Bhathena J, Prakash S. 884-893. 1st Annual Meeting of the Natural. Canadian Journal of Physiology and Pharmacol-ogy. 2007;(9).
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