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Kinetics of Hydrolyzing Isolated Soy Protein by an Endopeptidase and its Conceptual Application in Process Engineering

zebin wang ,
zebin wang
Contact zebin wang

Discovery Research, Solae, LLC, 4300 Duncan Ave, St Louis United States

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Jason Lombardi ,
Jason Lombardi

Discovery Research, Solae, LLC, 4300 Duncan Ave, St Louis, United States

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Jessica Shaffer ,
Jessica Shaffer

Discovery Research, Solae, LLC, 4300 Duncan Ave, St Louis, United States

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Ted Wong
Ted Wong

Discovery Research, Solae, LLC, 4300 Duncan Ave, St Louis, United States

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Published: 18.04.2012.

Volume 1, Issue 1 (2012)

pp. 26-32;

https://doi.org/10.7455/ijfs/1.1.2012.a3

Abstract

A response study and the effects of different parameters (pH, temperature and enzyme dose) on kinetics of isolated soy protein hydrolysis by a trypsin-like endopeptidase (TL1) were conducted. Degree of hydrolysis (%DH) data varied at different times under different hydrolysis conditions. Fitting the kinetics data to Michaelis-Menten kinetics model did not result in reasonable kinetic parameters, which implied that Michaelis-Menten kinetics was invalid for such a hydrolysis process. A kinetics model proposed by (Gonzalez-Tello, Camacho, Jurado, Paez, & Guadix, 1994) was found to fit the kinetics curve well and resulted in acceptable model parameters. A simple simulation example was performed to demonstrate the concept of how the kinetics equation could be applied in process engineering.

Keywords

isolated soy protein,

protein hydrolysis,

hydrolysis kinetics,

Trypsin-like endopeotidase,

process simulation

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