In this study, the retention of vitamin C in fresh broccoli stored at different temperatures (i.e. chiller, room, cooking, and roasting or baking; 5-120oC) was investigated. The thermal stability of vitamin C in broccoli was analysed at 5, 20, 45, 60, 70, 80, 110, and 120oC. The vitamin C content was measured by the indophenol titration method. Vitamin C was affected negatively at all stored temperatures. The degradation of vitamin C was modelled by first-order reaction kinetics and the reaction rate constants were observed as 9.03Ö10−8 and 5.65Ö10−3 s −1 when stored at 5oC and 120oC, respectively. The activation energy was estimated as 74.2 kJ/mol within the temperature range used in this study. The lowest decay of vitamin C was observed during the chilling condition. The data on retention of vitamin C in broccoli could be used to determine their stability, when stored as raw, and when heated at different temperatures.
Aoac. Association of official analytical chemists. Official methods of analysis of the AOAC. AOAC. 1990.
2.
Ariahu C, Abashi D, Chinma C. Kinetics of ascorbic acid loss during hot water blanching of fluted pumpkin (telfairia occidentalis) leaves. Journal of Food Science and Technology-mysore. 2011. p. 454–9.
3.
Awagu E, Ekanem E, Kolo A, Adamu M. Kinetic modeling of vitamin c (ascorbic acid) degradation in blanched commonly consumed salad vegetables using computer simulation analysis. Journal of Applied Chemistry. 2017. p. 59–66.
4.
Bergquist S, Gertsson U, Olsson M. Journal of the Science of Food and Agriculture. 2006. p. 346–55.
5.
Calligaris S, Manzocco L, Lagazio C. Modeling shelf life using chemical, physical, and sensory indicators. Shelf life assessment of food. 2012. p. 75–126.
6.
Charlton K, Patrick P, Dowling L, Jensen E. Ascorbic acid losses in vegetables associated with cook-chill food preparation. South African Journal of Clinical Nutrition. 2004. p. 56–63.
7.
Courtois F, Vedrenne L, George S, Dominguez-Perles R, Martinez-Ballesta C, Carvajal M, et al. Mathematical modelling of some nutrient losses during heat treatment of stewed apples. Czech Journal of Food Sciences. 2009. p. 383-C392.
8.
Favell D. A comparison of the vitamin c content of fresh and frozen vegetables. Food Chemistry. 1998. p. 59–64.
9.
Goncalves E, Abreu M, Brandao T, Silva C. 23rd International-Institute-of-Refrigeration(IIR) International Congress of Refrigeration. International Journal of Refrigerationrevue Internationale Du Froid. 2011. p. 2136–44.
10.
Hal P, Bosschaart C, Twisk C, Verkerk R, Dekker M. Kinetics of thermal degradation of vitamin c in marula fruit (sclerocarya birrea subsp caffra) as compared to other selected tropical fruits. LWT-Food Science and Technology. 2012. p. 188–91.
11.
Hamad Q. Spectrophotometric determination of total vitamin c in some fruits and vegetables at koya areakurdistan region/iraq. Kirkuk University Journal for Scientific Studies. 2009. p. 46–54.
12.
El-Ishaq A, Obirinakem S. Effect of temperature and storage on vitamin c content in fruits juice. 2015. p. 17–21.
13.
Ismail F. Determination of water soluble vitamin in fruits and vegetables marketed in sindh pakistan. 2013.
14.
Jain H, Mulay S. A review on biological functions and sources of anti-scorbutic factor. 2014.
15.
Jirasatid S, Noipant P. Burapha University International Conference. 2015.
16.
Kumar G, Kumar A, Raghu K, Manjappa P, S. Determination of vitamin c in some fruits and vegetables in davanagere city,(karanataka)-india. International Journal of Pharmacy & Life Sciences. 2013.
17.
Mapson L. Vitamin in fruits. The biochemistry of fruits and their products. 1970. p. 369–86.
18.
Masamba K, Mndalira K. Vitamin c stability in pineapple, guava and baobab juices under different storage conditions using different levels of sodium benzoate and metabisulphite. African Journal of Biotechnology. 2013. p. 186–91.
19.
Munyaka A, Makule E, Oey I, Van Loey A, Hendrickx M. Thermal stability of l-ascorbic acid and ascorbic acid oxidase in broccoli (brassica oleracea var. italica). Journal of Food Science. 2010. p. 336-C340.
20.
Nath A, Bagchi B, Misra L, Deka B. Changes in post-harvest phytochemical qualities of broccoli florets during ambient and refrigerated storage. Food Chemistry. 2011. p. 1510–4.
21.
Nisha P, Singhal R, Pandit A. A study on degradation kinetics of riboflavin in green gram whole (vigna radiata l.) Food Chemistry. 2005. p. 577–82.
22.
Oyetade O. Stability studies on ascorbic acid (vitamin c) from different sources. 2012. p. 20–4.
23.
Phillips K, Council-Troche M, Mcginty R, Rasor A, Tarrago-Trani T, M. Stability of vitamin c in fruit and vegetable homogenates stored at different temperatures. Journal of Food Composition and Analysis. 2016. p. 147–62.
24.
Polinati R, Kremer Faller A, Fialho E. The effect of freezing at-18 degrees c and-70 degrees c with and without ascorbic acid on the stability of antioxidant in extracts of apple and orange fruits. International Journal of Food Science and Technology. 2010. p. 1814–20.
25.
Rahman M, Al-Rizeiqi M, Guizani N, Al-Ruzaiqi M, Al-Aamri A, Zainab S. Effect of some cooking methods on natural antioxidants and their activities in some brassica vegetables. Journal of Food Science and Technology-mysore. 2015. p. 697–703.
26.
Shobham K, Mudhavath M, Sukumaran M. Effect of Microwave and Pressure Cooking on Stability of Vitamin C in Some Selected Vegetables. International Journal of Current Microbiology and Applied Sciences. 2017. p. 2391–7.
27.
Spinola V, Mendes B, Camara J, Castilho P, Spinola V, Mendes B, et al. An improved and fast uhplc-pda methodology for determination of l-ascorbic and dehydroascorbic acids in fruits and vegetables. evaluation of degradation rate during storage. Analytical and Bioanalytical Chemistry. 2012. p. 489–95.
28.
Suresh S, Al-Habsi N, Guizani N, Rahman M. Thermal characteristics and state diagram of freeze-dried broccoli: Freezing curve, maximal-freezeconcentration condition, glass line and solids-melting. Thermochimica Acta. 2017. p. 129–36.
29.
Venkatesh J, Park S. Role of lascorbate in alleviating abiotic stresses in crop plants. Botanical Studies. 2014. p. 55.
30.
Wang J, Law C, Mujumdar A, Xiao HW. The degradation mechanism and kinetics of vitamin c in fruits and vegetables during thermal processing. 2017.
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