Optimization of the Extraction of Antioxidants and Caffeine from Maté (Ilex paraguariensis) Leaves by Response Surface Methodology,
National University of Misiones
, Posadas
, Argentina
Optimization of the Extraction of Antioxidants and Caffeine from Maté (Ilex paraguariensis) Leaves by Response Surface Methodology,
National University of Misiones
, Posadas
, Argentina
Optimization of the Extraction of Antioxidants and Caffeine from Maté (Ilex paraguariensis) Leaves by Response Surface Methodology,
National University of Misiones
, Posadas
, Argentina
Optimal conditions for the industrial extraction of total polyphenols from maté (Ilex paraguariensis) were determined using response surface methodology, with two independent variables: ethanol percentage of the extraction solution and liquid to solid ratio. Response variables were total polyphenol content, antioxidant capacity, concentration of total polyphenols and caffeine content.The optimal conditions found were a liquid to solid ratio from 8 - 9 w w-1 and ethanol percentage of the extraction solution from 30 -50 % w w-1. Under these conditions the main predicted values corresponding to leaf extracts were 40 μg chlorogenic acid equivalents mL-1 of original extract, 13 g chlorogenic acid equivalents per 100 g dry matter for total polyphenol content, 22 g Trolox equivalents and 15.5 g ascorbic acid equivalents per 100 g dry matter for antioxidant capacity. The total polyphenol content of twig extracts was 36% lower than that in the leaf extracts.
Pagliosa C, Vieira M, Podesta R, Maraschin M, Bertello Zeni A, Amante E, et al. Methylxanthines, phenolic composition, and antioxidant activity of bark from residues from mate tree harvesting. 2010;
2.
Ijfs A. 2013;69–80.
3.
Zhou K, Yu L. Effects of extraction solvent on wheat bran antioxidant activity estimation. 2004;(7):717–21.
4.
Yao L, Jiang Y, Caffin N, Arcy D, Datta B, Liu N, et al. Phenolic compounds in tea from Australian super-markets. Food Chemistry. 2006;(4):614–20.
5.
Wijngaard H, Brunton N. The optimisation of solid-liquid extraction of antioxidants from apple pomace by response surface methodology. Journal of Food Engineering. 2010;(1):134–40.
6.
Wettasinghe M, Shahidi F. Evening primrose meal: A source of natural antioxidants and scavenger of hydrogen peroxide and oxygen-derived free radicals. Journal of Agricultural And Food Chemistry. 1999;(5):1801–12.
7.
Turkmen N, Sari F, Velioglu Y. Effects of extraction solvents on concentration and antioxidant activity of black and black mate tea polyphenols determined by ferrous tartrate and Folin-Ciocalteu methods. Food Chemistry. 2006;(4):835–41.
8.
Spigno G, Trarnelli L, De Faveri D. Effects of extraction time, temperature and solvent on concentration and antioxidant activity of grape marc phenolics. Journal of Food Engineering. 2007;(1):200–8.
9.
Santos D, Veggi P, Meireles M. Optimization and economic evaluation of pressurized liquid extraction of phenolic compounds from jabuticaba skins. Journal of Food Engineering. 2012;(3):444–52.
10.
Sambiassi C, Escalada A, Schmalko M. Extraction optimization of soluble compounds of yerba mate. Brazilian Archives of Biology and Technology. 2002;(2):189–93.
11.
Sairro S, Gosmann G, Saffi J, Presser M, Richter M, Bergold A. Characterization of the constituents and antioxidant activity of Brazilian green tea (Camellia sinensis var. assamica IAC-259 cultivar) extracts. Journal of Agricultural and Food Chemistry. 2007;(23):9409–14.
12.
Rostagno M, Palma M, Barroso C. Pressurized liquid extraction of isoflavones IJFS April. Analytica Chimica Acta. 2004;(2):169–77.
13.
Ramallo L, Lovera N, Schmalko M. Effect of the application of intermittent drying on Ilex paraguariensis quality and drying kinetics. Journal of Food Engineering. 2010;(2):188–93.
14.
Pineda Rivelli D, Da Silva V, V, Ropke D, Varella C, D, et al. Simultaneous determination of chlorogenic acid, caffeic acid and caffeine in hydroalcoholic and aqueous extracts of Ilex paraguariensis by HPLC and correlation with antioxidant capacity of the extracts by DPPH reduction. Brazilian Journal of Pharmaceutical Sciences. 2007;(2):215–22.
15.
Food Chemistry. (1):173–8.
16.
Astill C, Birch M, Dacombe C, Humphrey P, Martin P. Factors affecting the caffeine and polyphenol contents of black and green tea infusions. Journal of Agricultural and Food Chemistry. 2001;(11):5340–7.
17.
Lapornik B, Prosek M, Wondra A. Comparison of extracts prepared from plant by-products using different solvents and extraction time. Journal of Food Engineering. 2005;(2):214–22.
18.
Khokhar S, Magnusdottir S. Total phenol, catechin, and caffeine contents of teas commonly consumed in the United Kingdom. Journal of Agricultural and Food Chemistry. 2002;(3):565–70.
19.
Jokić S, Velić D, Bilić M, Bucić-Kojić A, Planinić M, Tomas S. Modelling of the Process of Solid-Liquid Extraction of Total Polyphenols from Soybeans. Czech Journal of Food Sciences. 2010;(3):206–12.
20.
Argentine Institute of Standardization and Certification -Yerba mate: Determination of caffeine content. 2003;
21.
Holovatty S, Argüello B, Malee L. Proceedings of 4 th South American Congress of Yerba Maté. Posadas Argentina From. 2006;(8):127–31.
22.
Hayouni E, Abedrabba M, Bouix M, Hamdi M. The effects of solvents and extraction method on the phenolic contents and biological activities in vitro of Tunisian Quercus coccifera L. and Juniperus phoenicea L. fruit extracts. Food Chemistry. 2007;(3):1126–34.
23.
Hartwig V, Brumovsky L, Fretes M. A total polyphenol content of mate (Ilex paraguariensis) and other plants-derived beverages. Journal of Food Research. 2012;(3):3–58.
24.
Hartwig V, Brumovsky L, Fretes R, Sánchez Boado L. A novel procedure to measure the antioxidant capacity of Yerba maté extracts. 2012;126–33.
25.
Filip R, Lotito S, Ferraro G, Fraga C. Antioxidant activity of Ilex paraguariensis and related species. Nutrition Research. 2000;(10):80024.
26.
Dudonné S, Vitrac X, Coutiere P, Woillez M, Merillon JM. Comparative Study of Antioxidant Properties and Total Phenolic Content of 30 Plant Extracts of Industrial Interest Using DPPH, ABTS, FRAP, SOD, and ORAC Assays. Journal of Agricultural and Food Chemistry. 2009;(5):1768–74.
27.
Food Science. (1):240–8.
28.
Cacace J, Mazza G. Optimization of extraction of anthocyanins from black currants with aqueous ethanol. Journal of IJFS. 2003;69–80.
29.
Bravo L, Goya L, Lecumberri E. LC/MS characterization of phenolic constituents of mate (Ilex paraguariensis, St. Hil.) and its antioxidant activity compared to commonly consumed beverages. Food Research International. 2007;(3):393–405.
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