Optimization of the Extraction of Antioxidants and Caffeine from Maté (Ilex paraguariensis) Leaves by Response Surface Methodology

Vanessa Hartwig ,
Vanessa Hartwig
Contact Vanessa Hartwig

Optimization of the Extraction of Antioxidants and Caffeine from Maté (Ilex paraguariensis) Leaves by Response Surface Methodology, National University of Misiones , Posadas , Argentina

Miguel Eduardo Schmalko ,
Miguel Eduardo Schmalko

Optimization of the Extraction of Antioxidants and Caffeine from Maté (Ilex paraguariensis) Leaves by Response Surface Methodology, National University of Misiones , Posadas , Argentina

Stella Maris Alzamora ,
Stella Maris Alzamora

Industry Department, Faculty of Exact and Natural Sciences, University of Buenos Aires , Buenos Aires , Argentina

Luis Alberto Brumovsky
Luis Alberto Brumovsky

Optimization of the Extraction of Antioxidants and Caffeine from Maté (Ilex paraguariensis) Leaves by Response Surface Methodology, National University of Misiones , Posadas , Argentina

Published: 18.04.2013.

Volume 2, Issue 1 (2013)

pp. 69-80;

https://doi.org/10.7455/ijfs/2.1.2013.a6

Abstract

Optimal conditions for the industrial extraction of total polyphenols from maté (Ilex paraguariensis) were determined using response surface methodology, with two independent variables: ethanol percentage of the extraction solution and liquid to solid ratio. Response variables were total polyphenol content, antioxidant capacity, concentration of total polyphenols and caffeine content.The optimal conditions found were a liquid to solid ratio from 8 - 9 w w-1 and ethanol percentage of the extraction solution from 30 -50 % w w-1. Under these conditions the main predicted values corresponding to leaf extracts were 40 μg chlorogenic acid equivalents mL-1 of original extract, 13 g chlorogenic acid equivalents per 100 g dry matter for total polyphenol content, 22 g Trolox equivalents and 15.5 g ascorbic acid equivalents per 100 g dry matter for antioxidant capacity. The total polyphenol content of twig extracts was 36% lower than that in the leaf extracts.

Keywords

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