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Volume 13, Issue 2, 2024

Online ISSN: 2182-1054

Volume 13 , Issue 2, (2024)

Published: 18.10.2024.

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01.12.2017.

Professional paper

Extraction and characterisation of cellulose nanocrystals from pineapple peel

The potential of pineapple peel as a source of cellulose nanocrystals was evaluated. Peels skin from fresh-cut fruit was used as raw material. These residues were purified to remove pigments, lipids and hemicellulose, and a bleaching process for delignification was carried out for 4-6 h. All resulting products were characterised for their lignin, hemicellulose, cellulose and ash contents using standard techniques. Dry matter at the end was low (ca. 50%) compared with the raw material (ca. 90%). The process applied resulted in ca. 20% (m/m) of purified cellulose (ca. 80% purity), with ineligible levels of lignin and hemicellulose present, especially when using 6h of bleaching. The purified cellulose was subject to acid hydrolysis for nanocrystal extraction with two testing times, 30 and 60 minutes. These cellulose nanocrystals had small sizes (< 1000 nm), with high variability and negative zeta potential values. The time of extraction did not affect the nanocrystals’ chemical and physical properties. The use of 6 h of bleaching treatment during purification was shown to be more effective than 4 h. Pineapple peel was demonstrated to be a good source of cellulose for the production of cellulose nanocrystals.

Ana Raquel Madureira, Tuğba Atatoprak, Duygu Çabuk, Flávia Sousa, Robert C. Pullar, Manuela Pintado

18.04.2018.

Original scientific paper

Extraction and characterisation of cellulose nanocrystals from pineapple peel

The potential of pineapple peel as a source of cellulose nanocrystals was evaluated. Peels skin from fresh-cut fruit was used as raw material. These residues were purified to remove pigments, lipids and hemicellulose, and a bleaching process for delignification was carried out for 4-6 h. All resulting products were characterised for their lignin, hemicellulose, cellulose and ash contents using standard techniques. Dry matter at the end was low (ca. 50%) compared with the raw material (ca. 90%). The process applied resulted in ca. 20% (m/m) of purified cellulose (ca. 80% purity), with ineligible levels of lignin and hemicellulose present, especially when using 6h of bleaching. The purified cellulose was subject to acid hydrolysis for nanocrystal extraction with two testing times, 30 and 60 minutes. These cellulose nanocrystals had small sizes (< 1000 nm), with high variability and negative zeta potential values. The time of extraction did not affect the nanocrystals’ chemical and physical properties. The use of 6 h of bleaching treatment during purification was shown to be more effective than 4 h. Pineapple peel was demonstrated to be a good source of cellulose for the production of cellulose nanocrystals. 

Ana Madureira, Tugba Atatoprak, Duygu Cabuk, Flavia Sousa, Manuela Pintado, Robert C. Pullar

18.04.2018.

Original scientific paper

Optimization of osmotic dehydration of chestnut (Castanea sativa Mill.) slices using Response Surface Methodology

Osmotic dehydration of chestnut slices in sucrose was optimized for the first time by Response Surface Methodology (RSM). Experiments were planned according to a three-factor central composite design (α=1.68), studying the influence of sucrose concentration, temperature and time, on the following parameters: volume ratio, water activity, color variation, weight reduction, solids gain, water loss and normalized moisture content, as well as total moisture, ash and fat contents. The experimental data was adequately fitted into second-order polynomial models with coefficients of determination (R2 ) from 0.716 to 0.976, adjusted-R2 values from 0.460 to 0.954, and non-significant lacks of fit. The optimal osmotic dehydration process conditions for maximum water loss and minimum solids gain and color variation were determined by the “Response Optimizer” option: 83% sucrose concentration, 20 °C and 9.2 hours. Thus, the best operational conditions corresponded to high sugar concentration and low temperature, improving energy saving and decreasing the process costs.

Teresa Delgado, Bruna Paim, Jose Alberto Pereira, Susana Casal, Elsa Ramalhosa

01.12.2017.

Professional paper

Quality assessment and shelf life modeling of pulsed electric field pretreated osmodehydrofrozen kiwifruit slices

The objective of this workwas to investigate the potential use of pulsed electric field (PEF) in combination with osmotic dehydration (OD) as a pre-freezing step and to evaluate the effect on quality characteristics and shelf life of frozen kiwifruit. Peeled kiwifruit was subjected to PEF (1.8 kV/cm), sliced and treated in OD-solution [containing glycerol, maltodextrin, trehalose, ascorbic acid, calcium chloride, citric acid, sodium chloride; 1/5 (wfruit/wsolution)] for 30 and 60 min at 35 °C. Combined, PEF only and OD only treated samples as well as nontreated and blanched (80 °C, 60 s) samples were frozen and stored at constant (-5, -10, -15, -25 °C) and dynamic temperature conditions (-18 °C-3 d, -8 °C-2.5 d, -15 °C-3 d). Quality of frozen samples was evaluated by means of drip loss, colour, texture, vitamin C and sensory evaluation (1-9 scale); and shelf life (SL) was calculated. Nontreated and blanched samples presented high drip loss and tissue softening (instrumentally measured as Fmax decrease). The tissue integrity was well retained in all osmotically pretreated samples. PEF pretreatment caused increase of fruit whiteness (increase of L value) and yellowness (a and/or b value increase); SL calculation was based on colour change. All OD samples had high vitamin content (24.6 mg/100 g fresh material compared to 138-154 mg/100 g osmodehydrated material); PEF led to 93% (of the initial) vitamin retention; blanched samples showed the lowest retention (86.9% of the initial) (criteria for SL calculation). OD and combined PEF-OD treatment increased the shelf life of frozen kiwifruit (up to 3 times; based on sensorial criteria). The developed kinetic models for colour change, vitamin loss, and sensory quality deterioration were validated at dynamic temperature conditions. PEF pretreated OD (at significantly shorter time, 30 min compared to 60 min) kiwifruits retained optimum quality and sensory characteristics. PEF and OD could be used as a preprocessing step of good quality, longer shelf life kiwi sliced frozen products.

Efimia Dermesonlouoglou, Ismini Zachariou, Varvara Andreou, Petros S. Taoukis

18.04.2018.

Original scientific paper

Estimating the risk of phthalates exposure via tea consumption in general population

Four common phthalic acid esters (PAEs) levels in tea fusions samples prepared from three types of tea bags (green, black and white) of ten commercial brands were extracted from the infusions by a dispersive liquid-liquid micro extraction method and determined by GC-MS. PAEs were not found in white tea samples. Residue levels of total phthalic acid esters (TPAEs) in black and green teas showed no significant difference (median=367.5, Interquartile range=244.7-667.5 and median=381, Interquartile range=188.7-688.2µg/kg respectively). DEHP levels in green teas were significantly higher than those in black teas (Median= 93.5 and 204 respectively). Total phthalate esters (TPAEs) levels in flavored teas were about two-fold higher than in non-flavored teas. The four commercial brands tested contain significant levels of DEHP when compared to other brands. Essential oils and essences that were added to tea for improvement of color and taste could be the main sources of PAEs contamination.

If oral absorption of phthalates were assumed to be 100%, the maximum daily exposure levels to TPAEs via tea consumption (due to consumption of 5 cups of tea prepared from the tea containing the highest levels TPAEs) were estimated to be 230e−4 µg/kg bw/Day, which are far lower than the regulation levels set by the expert panels on regularly toxicity. 

Mohammad Mehdi Amin, Foruz Rastegari, Parinaz Poursafa, Karim Ebrahim

18.10.2018.

Original scientific paper

Consumers’ willingness to consume cassava leaves as a leafy vegetable in the Kumasi Metropolis, Ghana

This study employs the logit model to assess the determinants of consumers’ willingness to consume cassava leaves as a leafy vegetable in the Kumasi Metropolis of Ghana. A multistage sampling technique was used to select 180 respondents for the study. The study found that majority (76%) of the respondents had no knowledge of the nutritional value of cassava leaves, though they had consumed the product before. The empirical results showed that socioeconomic characteristics of respondents such as age, sex, household size and monthly income, as well as their perceptions on the attributes and use of cassava leaves as food have significant influence on willingness to consume cassava leaves as a leafy vegetable. There is the need to provide information on the nutritional benefits of cassava leaves to facilitate decision-making on its utilisation/consumption. Programmes aimed at promoting the consumption of cassava leaves should consider the significant variables that have influence on the consumption of the product.

Fred Nimoh, Godfred O. Asare, Ismael Twumasi, Richmond Anaman

18.04.2018.

Original scientific paper

Effects of heat treatment by immersion in household conditions on olive oil as compared to other culinary oils: a descriptive study

The objectives were to evaluate the properties of refined (ROO) and extra-virgin olive oil (EVOO) in their natural state (fresh) and after heating, while comparing them with each other and with refined soybean (SBO) and refined sunflower seed oil (SFO). The methodology was designed to simulate, in controlled laboratory conditions, the home-frying process, while evaluating fatty acid profile (fatty acid methyl esters were separated by gas chromatography), concentration of phenolic compounds (Gallic acid dosage), antioxidant activity (DPPH), and production of polar compounds (thin layer chromatography) before and after heating to 200 °C for six minutes. It was observed that, before and after heating, SBO and SFO are rich in polyunsaturated fatty acids (FA) and ROO and EVOO are rich in monounsaturated FA. Fresh or heated, ROO and EVOO do not have trans FA, which are present in SBO and SFO, and increase in SBO after heating (+ 32.8%). The concentrations of phenolic compounds are always higher in olive oils, despite the decrease that occurs after heating (-7.5% in the ROO and -24.6% in EVOO). Antioxidant activity is greater when olive oils are fresh and remains present in EVOO after heating. The concentration of polar compounds was similar for all oils after heating. In conclusion, ROO and EVOO are the richest in monounsaturated FA even after heating, with no production of saturated or trans FA. Despite losing some antioxidant activity, heated EVOO remains richer in monounsaturated FA than ROO, SBO and SFO in the fresh version. All oils suffer similar rates of degradation.

Carlos Nogueira-de-Almeida, Georgia A. de Castro

18.10.2018.

Original scientific paper

Antioxidant and antibacterial activities of exopolysaccharides produced by lactic acid bacteria isolated from yogurt

The objectives of this study were to optimize the conditions for cell growth and exopolysaccharides (EPS) production by using pure and mixed microbial cultures of Streptococcus thermophilus and Lactobacillus bulgaricus, and to evaluate the antioxidant and antibacterial activities of EPS in vitro. The mixed cultures of two strains showed a higher cell growth whereas the higher EPS production was detected in pure culture with S. thermophilus. The optimal medium were determined as follows (g/l): sucrose 50, yeast extract 10, KH2PO4 3 , MgSO4.7H2O 0.05 and pH initial 6.5 at 30°C. Under the optimized conditions, the values of dry cell weight (DCW) and EPS were 5.2± 0.8 g/l and 56.8 ± 0.62 mg/ml, respectively.  The EPS demonstrated a positive antioxidant potential on DDPH radical scavenging. The agar diffusion assay showed that purified EPS exhibited antibacterial activities against tested pathogens such as Escherichia coli ATCC 250922 and Staphyloccocus aureus ATCC 250923 at (62-1000) μg/mL. In conclusion, EPS have an antioxidant activity and could have applications in the food industry.

Benattouche Zouaoui, Djillali Bouhadi, Ghalem Bachir Raho

18.04.2018.

Original scientific paper

Effect of storage time and gamma irradiation on the chemical properties of olive (Olea europaea) oils

The objective of this study was to investigate the effects of gamma irradiation at doses of 0, 1, 2 and 3 kGy, and storage time of olive fruits for 0, 30 and 45 days on changes in chemical properties of olive oil during storage periods of 0, 6 and 12 months. The initial acidity value (AV), peroxide value (PV), Thiobarbituric Acid (TBA), phenolic content, iodine value (IV) and saponification value (SV) of virgin olive oil obtained from olives fruits immediately after harvest (at day zero) were 1.04%, 3.06 mEqO2 kg−1 oil, 0.025 mg MDA kg−1 oil, 314.71 mg gallic acid kg−1 oil, 93.38 gI2 100 g−1 oil and 194.88 mg KOH g−1 oil, respectively. In general, the AV and PV of olive oil was increased by gamma irradiation, while the phenolic and IV of olive oil was deceased by gamma irradiation and storage time. The TBA value and SV of olive oil was not significantly (p>0.05) changed by gamma irradiation.

Al-Bachir M.

18.04.2018.

Original scientific paper

Energy pattern and conservations of condiment produced from soybean (Glycine max)

Energy being one of the largest operating expenses in most organizations especially manufacturing and processing industries leading to considerable scope for energy conservation and hence cost. Information on energy utilization and conservation pattern were obtained based on time taken, number of person involved and sources of energy using standard energy equations. A total of 445.40 ± 17.32MJkg−1 where thermal energy (420MJ ≈ 94%) and manual energy (25.40MJ ≈ 6%) were the only forms of energy used during production process. Conservation approach I resulted in mean energy of 72.08 ± 1.73MJkg−1 where electrical energy, manual energy and thermal energy accounted for 1.75MJ (3%) 7.34MJ (10%) and 62.99MJ (87%) respectively. Conservation approach II reduced the energy further to 57.24 ± 1.73MJkg−1 as the operation was thermal energy dependent, followed by manual and electrical energy with energy values of 48.13, 7.33 and 1.78MJ equivalent to 84.10%, 12.80% and 3.10% accordingly. Conclusively, traditional method of processing utilized highest energy (445.40MJ) followed by conservation approach I (72.08MJ) and conservation approach II (57.24MJ) was least in energy demand. Conservation approach II permits energy conservation to be 87% as compared with traditional method.

ismaila anjorin, Rahman Akinoso, Mayowa S. Sanusi

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